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KMID : 1035320230500010059
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Korean Society for Dental Materials
2023 Volume.50 No. 1 p.59 ~ p.68
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A study on the effect of denture cleaning utilizing active micro-locomotion of diatom complex
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Lee Joo-Hun
Kong Hyun-Joon
Choi Ji-Won
Park Eun-Jin
Jang Hye-Rin
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Abstract
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This study compared the effectiveness of self-propelling diatom microbubblers to clean dental appliances with commercialdenture cleaning agents according to the Ministry of Food and Drug Safety’s guidelines. The microbubbler is made by dopingdiatoms with MnO2 nanosheets that can decompose hydrogen peroxide to generate oxygen bubbles. Artificial saliva is preparedin accordance with the criteria presented by the American Dentist Association, dispensed, and dried in 96 well plates. Experimentalgroups include 10-15% NaOCl (positive control), distilled water (negative control), diatom microbubbler A (Aulacoseira,MnO2-polydopamine (PDA)-A), diatom microbubbler M (Melosira nummuloids, MnO2-sugar (S)-M), Polident (GlaxoSmithKline,Dungarvan, Ireland), Dentfix-forte (Helago-Phama GmbH&Co, Parchim, Germany). After washing, absorbance (OD 600) wasmeasured. If the absorbance was 70% or higher, the condition was determined to have “cleaning power potency,”Statisticalsignificance was evaluated by one-way ANOVA and Bonferroni correction to compare cleaning effects among groups (p<0.05).
The average cleaning rates were 93.8±1.0% in NaOCl (positive control) and 79.1±1.5% in distilled water (negative control).
With the diatom microbubbler A, the average cleaning rate was 79.8±4.5% in the 3% H2O2 2 mg/mL, 64.7±5.5% in 6% H2O24 mg/mL, and 81.9±7.9% in 6% H2O2 4 mg/mL. The diatom microbubbler M group showed average cleaning rates of 88.5±3.6%in 3% H2O2 2 mg/mL, 75.8±4.0% in 6% H2O2 2 mg/mL, and 84.5±4.5% in 6% H2O2 4 mg/mL. Finally, conventional denturecleaning agents showed average cleaning rates of 88.1% in Polident and 83.3% in Dentfix-forte. The positive control grouphad significant differences from all experimental groups, but the negative control group showed significant differences onlyin A2 and A3, M1 and M2, M3, Polident, and Dentfix-forte (F=190.141, p <0.001). Among all groups except the positive controlgroup, MnO2-S-M mixed with 3% H2O2 2 mg/mL showed the highest cleaning rate. As the results of this study show, diatomcomplexes exhibit cleaning effects compatible with conventional denture cleaning agents. Further studies need to be conductedto narrow down the specific optimal conditions of diatom microbubblers and maximize the cleaning effect.
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KEYWORD
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Diatom microbubbler, Active micro-locomotion, Denture cleaning
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